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Such two-strand lesions are mostly generated by replication of DNA with one-strand lesions.
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Chance and the enormous variation generated by replication errors in millions of stem cell lineages may be sufficient for the selection of low frequency cancer phenotypes within a lifetime.
Single-stranded DNA fragments, generated by replication fork stall or enzymatic processing of broken DNA ends [ 8], bind and activate RecA [ 9].
Clearly, the process of repair takes place after the initial phase of BRCA1/BARD1 downregulation, most likely to repair DSBs generated by replication blocking lesions.
This indicates that homologous recombination is reactivated at later stage of the cellular response to MMS, most likely to repair DSBs generated by replication blocks.
Thus, our data do not corroborate the notion of instability being generated by passive replication through an unfired origin.
Intrachromosomal dicentrics can also be generated by processing or replication of inverted repeats (Lobachev et al., 2002; Narayanan et al. 2006; VanHulle et al. 2007).
However, using a pulse-chase system to label the progeny of pre-existing β cells, Dor et al. [ 28] provided evidence that all or nearly all new β cells in the adult pancreas including those generated after pancreatectomy are derived by replication of pre-existing β cells.
Differences in genome signature between different organisms can be attributed to differences in context-dependent mutation rates generated by the replication-repair system and differences in efficiency of the replication machinery on different sequences.
Additionally, NHEJ cannot deal with one-ended DSBs generated by breakage of the replication fork.
Nanotechnology is portrayed as the cause of a future ecological catastrophe, generated by the self-replication of lethal nano-robots.
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