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Alternatively, hydrogen can be generated by in situ gasification of bitumen.
We suggest that TMD method may help in discriminating between ligands generated by in silico docking.
The active phase of the catalyst was generated by in situ carburization of MoO3/SO42− ZrO2.
HPLC water grade was generated by in house Milli-Q plus purification system (Millipore, Waters, USA).
RNA transcripts of each UCP generated by in vitro transcription were used to validate the specificity and sensitivity of the assay.
The synergistic effects of scaffold properties and hydrodynamic environment generated by in vitro rocking culture were also investigated using static cultures as control.
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The ctcH and ctcJ deletion mutants of S. aureofaciens DT1 were generated by in-frame deletion (Fig. 4a) and confirmed with PCR (Fig. 4b).
The AB-plots were generated by in-house developed software programmed in Matlab (The MathWorks Inc., Novi, MI, USA).
Antibody variants were either de novo synthesized (GeneArt) or generated by in-house site-directed mutagenic PCR and confirmed by sequencing.
VHL-UbΔGG and VHL-SUMO1ΔC4 were generated by in-frame ligation of BamHI/XbaI PCR fragments to the downstream of FLAG-VHL K171R). FLAG-VHL K171R
Peptides, generated by in-gel digestion with trypsin, were separated by liquid chromatography followed by double mass spectrometry (LC/MS/MS) and finally sequenced by Edman degradation.
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