Sentence examples for generated by cyclin from inspiring English sources
Together, our observations demonstrate that mono-phosphorylated Rb, generated by cyclin D Cdk4/6 complexes, is the functionally active Rb isoform present in early G1 phase.
This experiment showed that mono-phosphorylated Rb, generated by cyclin D Cdk4/6, was functional and required to prevent inappropriate cell cycle progression and E2F transcription downstream of an ATR/ATM DNA damage response.
These observations fundamentally change our understanding of G1 cell cycle progression and show that mono-phosphorylated Rb, generated by cyclin D Cdk4/6, is the only Rb isoform in early G1 phase.
These are apparently generated by cyclin-dependent kinases through stepwise phosphorylation of S23, T60 and S64 in the cell cycle.
Human primary myoblasts obtained from a healthy donor (KM109) [ 42], immortalized myoblast cell line (7304-1) generated by expressing telomerase (hTERT) and cyclin-dependent kinase 4 [ 43], and DMD patient derived myoblasts with a deletion in exon 51-55 DL589.22) [ 7] were used in this study.
In their study, only the high-avidity CTL clones, generated by limiting dilution, could kill the cyclin D1-expressing MDA-MB-231 cells, while the CTL clone with low-avidity failed to do so.
Polyadenylated cyclin D1 mRNA was generated by using a poly(A) tailing kit (Ambion).
Uncapped Cyclin D1 mRNA was generated by MEGAscript kit containing T7 RNA polymerase (Ambion, Austin, TX).
Capped cyclin D1 mRNA was generated by using a mMESSAGE mMACHINE high yield capped RNA transcription kit containing T7 RNA polymerase (Ambion).
The 9p21.3 region encodes three tumor suppressor genes that play key roles in cell cycle inhibition, senescence and stress-induced apoptosis: CDKN2A/p16 INK4A (cyclin-dependent kinase inhibitors 2A), p14 ARF (alternative transcript generated by alternative exon 1 of CDKN2A/p16 INK4andand CDKN2B/p15 INK4B (cyclin-dependent kinase inhibitors 2B) (37).
Similarly, mutant cyclin B 3′-UTRs were generated by overlap extension PCR method and cloned into vector pGL3.
