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As expression levels are unpredictable following pronuclear microinjection, multiple transgenic lines must be generated bred and analysed for expression.
AlbFxn−/− mice were generated, bred and verified as described previously [ 12].
12 17 All mice were generated, bred and maintained under specific pathogen-free conditions at the animal facility of the Erasmus Medical Centre (Rotterdam, The Netherlands).
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O'Neill et al. (2010) illustrates these trade-offs within a summary of the evolutionary history of Bos taurus cattle, as adaptation to stress-free temperate conditions generated breeds with little resistance to diseases common in tropical regions.
Transgenic mice expressing a subphysiological level of a p53(R172L) minigene (PB-p53(R172L)) in the prostate epithelium were generated and bred to the transgenic adenocarcinoma mouse prostate (TRAMP) model of prostate cancer.
Transgenic mice expressing either the wild type H-2Kb MHC I allele (KbWT), or H-2Kb containing a single point mutation substituting a phenylalanine residue for the conserved tyrosine in exon 6 of the molecule (ΔY) were generated and bred onto a H-2K background by backcrossing transgenic founders with C3H/He mice.
LKB1fl/fl mice were generated and bred as previously described 34.
Cordula Rumig, Günter Hämmerling and Bernd Arnold generated and bred the Hsd17b10 conditional knock-out mice.
Results Heterozygous Tp53 LSL3R270H/+ Nkx3.1-Cre mice with prostate-specific expression of the Tp53 R270H mutation were generated and bred onto a fully congenic FVB/NJ background.
The homozygous mutant mice lacking the PBP/MED1 in hepatocytes (PBP/MED1ΔLiv) were generated and bred in our laboratory as described elsewhere (27).
A new mouse strain (MMTV-Luc2 mice) expressing Luc2 luciferase primarily in mammary tissue in females, with low-level background expression in internal organs, was generated and bred to homozygosity.
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