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In contrast, using the same techniques, gfp transcripts and GFP signal could be detected in all the mutants generated bearing the GFP generator under the control of a constitutive synthetic promoter (based on the photosensitive promoter of the psbA2 gene of Synechocystis).
A transgenic mouse was generated bearing human FcgRIIa.
Two pairs of microparticle types were generated bearing complementary DNA.
26 As already mentioned above, an alternative reaction pathway has been identified for the ODH-process of the [V2P2O10].+ cluster in which a structural isomer of 10, that is, 10 – 2 in Figure 3, is generated bearing two P OH moieties instead of the P OH/V OH groups as in 10.
Similar to the [V2P2O10] .+/C2H4couple, an alternative pathway for the oxidative dehydrogenation in the reaction of [V2P2O10].+ with C2H6 has been found in which the more stable product ion 10 – 2 is generated bearing two P OH units instead of one V OH and one P OH group.
For the deletion of the eight main hexose transporter genes (HXT1– 7 and GAL2) in S. cerevisiae, four deletion constructs were generated bearing either the kanMX, zeoMX, hphMX, natMX [ 42, 43].
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To validate the proposed method, two cases were considered by using the degradation data generated by bearing testing rig.
In order to precisely determine which cells undergo apoptosis in the different ablated embryos, we generated mice bearing both the Dbx1loxP-stop-loxP-DTA and the ROSA26loxP-stop-loxP-YFP alleles and mated them with Cre-expressing lines.
To test these possibilities, we generated mice bearing a mutated allele of Iqcg lacking exon 6 (Iqcg KO/KO).
To determine whether the proviral effect of TNF-α is specific for HCV, we generated pseudoparticles bearing the surface glycoproteins of Lassa, measles, and vesicular stomatitis virus (VSV).
We also generated mutants bearing leucine-to-proline substitutions at position 7 in one or both SBSs (arrow in Figure 4A; see Additional file 2 for details).
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