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MG-RAST generated assignments of protein-coding nucleotide sequences to 29 broad functional categories of SEED subsystems and 637 narrow SEED subsystems (Level 1 and Level 3, respectively).
For example, ML-corrected distances to MRCA in gag generated assignments that were not congruent with other distance measurements in 7 of 25 cases of maximum distances and in 15 of 25 mean distances (see Cumulative Preliminary Summary discussion and Summary Table below).
We generated assignments with taxator-tk, CARMA3 and MEGAN4/5 under equivalent conditions, once with nucleotide and once separately with protein local alignments, and compared them with both Kraken and the published PhyloPythiaS assignments (Supplementary Fig. S21).
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In generating assignments we made extensive use of structure models, especially those of Gutell et al. for rRNAs [ 25].
The ISO solves a constrained optimization problem (linear programming in this case) in order to find (underline {S} = [S_{1}, ldots, S_{|G|}]), the optimum power generating assignment for the generators, and (underline {theta } = [theta _{1}, ldots, theta _{|B|}]), the voltage angles on the buses, which minimize the cost charged by the generators.
Her status as a finalist secured her professional representation, which quickly generated modeling assignments.
Phylogenetic analysis using the 94 nt ORF1b gene sequence spanned by the nPCR primers generated genotype assignments that were consistent with those obtained using the extended 671 nt sequence used for the initial identification of genotypes.
We generated COG assignments of the translated gene products by conducting a BLASTP search against the COG reference data set.
The co-crystal structure of the IS 50R transposase binding the ends of the Tn 5 transposon generated function assignments for numerous residues of this protein [ 9].
Proteomes deposited in the database were searched against the HMM libraries using the iterative Sequence Alignment and Modeling System (SAM) method [ 83], which has generated FSF assignments covering on average ~ 60% of amino acid residues of individual proteomes [ 82].
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