Sentence examples for generated as a fusion from inspiring English sources

Recombinant mouse ADAM2 (fertilin β) disintegrin domain was generated as a fusion protein with glutathione S-transferase (GST) as previously described [11].

ORCTL3-ECFP was generated as a fusion construct with a pcDNA3 vector backbone.

The kanamycin cassette for fosmid extension needs to be generated as a PCR fusion product [6] with the genomic DNA piece that will be added to the fosmid DNA.

DNMT3A and 3B have been shown to interact with normal TFs [ 167] and abnormal versions generated as a result of gene fusions [ 168].

In some instances such as chronic myelogenous leukemia (CML), the gene fusion generated as a result of the breakage rejoining event underpinning the translocation formed a new oncogenic BCR-ABL gene fusion (Melo 1996).

The latter represents a leukemogenic fusion protein generated as a consequence of a chromosomal translocation found in more than 90% of patients with chronic myeloid leukaemia (CML).

Id2-3XFlag fusion proteins were generated as a means for identifying exogenous Id2 from endogenous Id2.

As illustrated in Figure  3, fusion events allow mixing of oxidatively damaged mitochondrial contents generated as a byproduct of respiration.

Therefore, to determine the required SH3 domains for the Caskin1/Nck interaction, full length Nck, the three SH3 domains together (Nck-SH3-all), and individual SH3 domains (SH3/1, SH3/2 and SH3/3) were generated as GST fusion proteins.

Two notable exceptions, UBI2 and UBI3, which show increased expression in R57 relative to the WT (2.7- and 2.8-fold, respectively; Additional file 4: Data S2), encode ubiquitin fused to ribosomal proteins [ 31] and are responsible for generating ubiquitin as a fusion protein that is then cleaved to yield free ubiquitin by deubiquitinases.

Portions of these two ZNFs (Figure 2A) corresponding to the KRAB domains were generated as recombinant GST fusion proteins and were incubated with nuclear extract from K562 cells.