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It was generated as a control for cell lines expressing a gene of interest from pUHD10S.
A global inferred network was generated as a control for module-specific relationships, prior to generating the module-specific inference.
A construct encoding the corresponding his-tagged version of the wild type enzyme (WT-his) was generated as a control.
A random 6 Mb genome with 50% G+C content was generated as a control and demonstrated to have no oligonucleotide biases at a χ2-value of 100.
Mosaic mice were generated as a control to evaluate the effect of presence and absence of p120 within the same tissue.
The Z. mobilis parental and disruptant strains carrying pZA22 were generated as a control.
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U2M.L (universally unmethylated DNA) was generated as a negative control by amplifying the same DNA used to generate the positive control DNA using the GenomiPhi kit (GE Healthcare) as described by the manufacturer.
UM.L (universally methylated DNA ) was generated as a positive control by treating normal peripheral blood leukocyte (PBL) DNA with the CpG methylase M.SssI (New England Biolabs) [25].
Transmission electron microscopy (TEM, Fig. 3A and B) revealed that MstnPP soluble aggregates exhibit a morphology and size similar to that documented for amyloid protofibrils from a number of other proteins such as lysozyme [59], [60] and HypF-N [61] as well as the insulin protofibrils generated as a positive control (Fig. 3C) using a well-established protocol [39], [62], [63].
A stable Daoy cell line expressing a non-silencing shRNAmir (Open Biosystems) was also generated as a negative control.
An additional A549 cell line stably expressing the TAP tag only (TAP only) was generated as a negative control.
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