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Nonetheless, understanding how positional information is generated and maintained, as well as how such information is coupled to intracellular GRN dynamics, are key to understanding pattern formation during development.
Batf3−/− mice were both generated and maintained as breeding colonies in Harvard Medical School facility.
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Mated females were generated and maintained as for lifespans.
MDCK E-cadherin-RFP stable cell lines were generated and maintained as described [43], [44].
Cdk5-deficient mice were generated and maintained as described previously [37], [38].
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Our results demonstrated that human iPS cells can be generated and maintained on autologous fibroblasts as feeder layers.
asm−/−/ldlr−/−, a double knockout mouse colony, was generated and maintained in our laboratory as described previously by litter mating of asm+/−/ldlr−/− animals [ 13].
The second mutant allele of Ildr1 used in this study, Ildr1 tm1(KOMP)Wtsi, designated here as Ildr1 k−/−, was generated and maintained on a C56BL/6N background as part of The Wellcome Trust Sanger Institute Mouse Genetics Projects (project ID: 31366) (56, 56).
AhCre + Apc+/+ and AhCre + Apcfl/fl mice were generated and maintained on an outbred background as previously described [ 1].
Knockin mice in which the LKB1 farnesylated Cys is changed to serine to abolish prenylation were generated and maintained on an inbred C57BL/6J background as shown in Figure 2(A).
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CEO of Professional Science Editing for Scientists @ prosciediting.com