Sentence examples for generated and confirmed from inspiring English sources

The tir1-1/afb5 double mutant was generated through a cross of the two single mutant lines, and homozygous lines were generated and confirmed for tir1-1 using CAPS marker [27] and for afb5, using primers described above.

R26 F133V/+ mice were generated and confirmed though standard genotyping.

RS, MJ, TM, and BW generated and confirmed overexpression and insertion lines.

For each genotype, two plasmids were generated and confirmed by direct sequencing.

A total of 11 independent lines which overexpressed GmPIP1 6 were generated and confirmed by semi-qRT PCR.

For sequencing, independently amplified polymerase chain reaction products were generated and confirmed by automated sequencing with the ABI Prism BigDye Terminator Cycle Sequencing Kit (Perkin Elmer).

Our approach inserts additional plan operators that generate and confirm speculative results, while preserving the safety and fairness of overall execution.

A list of primers used to generate and confirm the allelic replacement mutants is provided (Table 3).

A 5′and 3′-RACE sequence-walking strategy was used to generate and confirm sequences of the ends of both RNAs.

cDNA microarray, real-time quantitative PCR, and immunohistochemistry on tissue microarrays were used to generate and confirm a gene signature associated with tamoxifen failure.

The Greek and Swedish cohorts of patients used to generate and confirm this risk stratification system are indicative of the local epidemiology of sepsis in Greece and Sweden as defined after comparisons with published series of Greek and Swedish patients [ 20- 22].