Sentence examples for generated and bred from inspiring English sources

Transgenic mice expressing a subphysiological level of a p53(R172L) minigene (PB-p53(R172L)) in the prostate epithelium were generated and bred to the transgenic adenocarcinoma mouse prostate (TRAMP) model of prostate cancer.

Transgenic mice expressing either the wild type H-2Kb MHC I allele (KbWT), or H-2Kb containing a single point mutation substituting a phenylalanine residue for the conserved tyrosine in exon 6 of the molecule (ΔY) were generated and bred onto a H-2K background by backcrossing transgenic founders with C3H/He mice.

Cordula Rumig, Günter Hämmerling and Bernd Arnold generated and bred the Hsd17b10 conditional knock-out mice.

LKB1fl/fl mice were generated and bred as previously described 34.

Results Heterozygous Tp53 LSL3R270H/+ Nkx3.1-Cre mice with prostate-specific expression of the Tp53 R270H mutation were generated and bred onto a fully congenic FVB/NJ background.

The homozygous mutant mice lacking the PBP/MED1 in hepatocytes (PBP/MED1ΔLiv) were generated and bred in our laboratory as described elsewhere (27).

These phenotypic results were obtained only 6 weeks after embryo injections were performed, in contrast to years it would have taken to generate and breed triple homozygotes from individually targeted ES cells.

Founders were generated and successfully bred to establish two independent lines (Brk83 and Brk); transgene presence was verified by PCR.

Human CD4/CCR5/Cyclin transgenicnic mice were generated individually and bred into double or triple transgenic lines.

Gal3−/− mice were previously generated as described and bred on the C57BL/6 mouse background for nine generations48.

RAGE-deficient (rage −/− ) [ 11] and tlr4 −/− mice [ 12], backcrossed >10 times to a C57BL/6 background were generated as described and bred in the animal facility of the Academic Medical Center (Amsterdam, the Netherlands).