To establish the common marmoset (Callithrix jacchus) as a model for FTLD, we generated a stereotaxic injection-based marmoset model of FUS-silencing.
Subcutaneous tumors were generated by an injection of 1 × 10 cancer cells/mouse in a mixture of phosphate-buffered saline (without magnesium or calcium) and BD Matrigel BD BiosciencesSan Jose, CA CA, USA) at a 1 1 ratio in the right flank.
The second single-longitudinal-mode pump beam centered near 561 nm is generated using an injection-seeded optical parametric oscillator, consisting of two non-linear β-BBO crystals, pumped using the third harmonic output (∼355 nm) of the same Nd YAG laser.
Point defect engineering techniques, with light energetic ion implants, have been applied to generate an ISi uniform injection in the electrically active layer.
AgNO3 injection generates an acute phase response with striking increase of SAA production within 24 hours.
Functional expression of Kir2.1 channels was assessed in isolated lumbar motoneurons by recording whole cell currents generated by injection of an 850 ms-voltage ramp from −130 to +20 mV (Fig. 4C & D).
The Notch-responsive GFP reporter line, Tg EPV.Tp1-Mmu.Hbb EGFP) ia12 (12xNRE:EGFP) was generaTg EPV.Tp1-Mmu.Hbb EGFPonstruct composed of sia12opies of the Epstein-Barr Virus Tp1 enhancer, each containing twasRbp-Jk bindingeneratedfor a total of 12 Notch-responsive elements, placed in front of a murine byta-globinjectionprofoter driving EGFP (Parsons et al., 2009).
We noted that transgenic animals generated by injection of a high concentration (50 mg/liter) of the ABU-14::GFP transgene were small and pale, and often arrested as young larva or required over a week to reach adulthood (in comparison to two days for wild-type animals).
In addition, Patlak analysis, a kinetic linearized model [16] for irreversible tracer uptake, was applied to the interval 10 to 60 min post injection to generate an image of net influx rate [K i] of FDG, which is proportional to the metabolic rate of glucose.
The results show that at the top of the reactor, flow from the 1 cm diameter injection tube generates an annular vortex of hydrogen; however below about 10 cm from the top, the hydrogen flow is well described by a parabolic velocity profile (Poiseuille).
