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To allow generation of a wide variety of TetR-expressing cell lines, we also generated an Entry vector with the TetR cDNA (pENTR/TetR) and recombined it with the pLenti CMV Blast DEST vector to make pLenti TetR Blast (not shown).
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When you lead an honor to drive out an opposing higher card and generate an entry for your partner, it is called a Deschapelles Coup.
The gateway attB-flanked PCR product was recombined with a donor vector containing the corresponding gateway attP signals in a BP reaction to generate an entry clone.
The oligoadenylated 3′ ends that we detect suggest that termination generates an entry site for the TRAMP and exosome complexes.
These data suggest that decapping by Dcp2 can often generate an entry site for Xrn2 to degrade aberrant RNA.
The purified PCR products were cloned by site-specific recombination into the donor vector pDONR221 to generate an entry clone.
Within endo/lysosomal compartments, host endosomal cysteine proteases (cathepsins) cleave the filoviral GP1 protein to generate an entry intermediate comprising an N-terminal GP1 fragment and GP2.
Briefly, a 300 to 400 base pair region was PCR amplified and TA-cloned (deoxythymidine, T, deoxyadenosine, A) into plasmid pCR/8GW/TOPO (Invitrogen, Grand Island, NY, USA) to generate an entry clone.
In the course of rRNA processing, cleavage within ITS1 might generate an entry point for the RNA-kinase Nol9, providing the 5′ phosphate and thus a favourable substrate for the subsequent 5′>3′ exonucleolytic activity (Stevens, 1980).
Step 2 : Entry clones were generated by BP reaction: Three PCR products from step 1and three donor vectors P4-P1r, P2-P3, P2-P3 were used in three separated BP recombination reactions between an att B-flanked DNA fragment and an att P-containing donor vector to generate an entry clone.
When a new token is generated, a new entry is added to this table.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com