To monitor localization of the endogenous protein in tissues, we generated an affinity-purified polyclonal antibody against FKH.
To determine when and where the GADR-1 protein accumulates, we generated an affinity-purified rabbit antibody to a 100-amino-acid part of the protein (see Materials and Methods) and used it to immunostain embryos.
An alternative or complementary way for Twitter to employ its Interest Graph, which ties people to interests, is to generate an affinity graph, which ties interests to other interests.
We expressed a His-tagged fragment of MBD1 (aa 1 69) in bacteria to generate an affinity matrix.
Purified His-p63TA1-115 was used to generate an affinity column by crosslinking it to Cyanogen bromide-activated Sepharose 4B beads, and serum containing the anti-p63TA antibodies was purified on it.
Here we report the isolation of a ∼ 45 kDa imidazoline binding protein from rabbit and rat brain using a high affinity ligand for the I2 subtype, 2-BFI, to generate an affinity column.
A 17-aa peptide (FTIQGHLWQSKKPTTPK) was used to immunize rabbits and to generate an affinity-purified anti-marshalin antibody (Covance, Denver, PA).
In conclusion, our study designed a method to prepare human recombinant HE4 with high yield and generated a high-affinity anti-HE4 monoclonal antibody that might have potential for basic research and clinical application.
We have generated a low-affinity photoconvertible probe, capable of reversibly binding to actin and thus allowing long-term photoactivated localization microscopy of the spine cytoskeleton.
We generated an RNase 7 affinity column to selectively isolate RNase 7 specific antibodies from the serum.
From this result, we demonstrated that our strategy was able to generate a high affinity molecule using only data in the training set.
