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Single stranded 454 libraries were then generated, amplified on beads with emulsion PCR, and sequenced as previously described [29].
Two new H3K4me3 gastrula ChIP-chip samples were generated, amplified using the T7 method and hybridized to the new promoter microarray design.
Model songs identical to those used in behaviour were generated, amplified and attenuated in steps of 1 dB using a Tucker-Davis system (Alachua, Florida).
Viruses were generated, amplified and titrated according to the manufacturer's manual.
The vectors encoding GFP from jellyfish, TGF-β1 and BMP-2 were generated, amplified, purified and used as described earlier [ 11, 16, 18].
Ambion WT Expression Kit (AMBION INC, NY, USA) generated amplified and biotylated sense-strand cDNA from the total RNA according to the manufacturer's protocol.
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Such RNA is generally processed through a multiple steps procedure to generate amplified amino allyl RNA (AA-aRNA) coupled with fluorescent dyes.
The presence of target triggers the opening of the hairpin to form target/aptamer complex and releases G-quadruplex sequence which can generate amplified colorimetric signals.
The timing of the pulses is designed to generate amplified spinning sideband manifolds which can be fitted to extract CSA parameters.
We provide an optimized experimental protocol that allows isolation of gene transcripts from fluorescent retrogradely labeled cell populations in fresh tissue, which can be used to generate amplified aRNA for microarray hybridization from as few as 50 laser microdissected cells.
The ExpressArt TR Micro Kit (Cat. No. 6199-A30, AmpTec) was used to synthesise double-stranded cDNA from 3 ug total RNA, which was subsequently used to generate amplified amino-allyl antisense RNA (aRNA) using the SuperScript Indirect RNA Amplification System (Cat. No. L1016-01, Invitrogen).
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