Sentence examples for generated a wild from inspiring English sources

We generated a wild type poliovirus population by transfecting in vitro transcribed RNA into HeLa S3 cells.

Up till then the renowned geneticist and plant breeder Charlie Rick (University of California, Davis) observed that crosses between wild and cultivated species generated a wild array of novel genetic variation in the offspring.

Five groups of mice were generated: (a) wild-type; (b) Rosa26 LacZ−stop−Met, (c) Nes-R26 Met, (d) SOD1 G93A (referred to as SOD), and (e) Nes-R26 Met ;SOD1 G93A (referred to as Nes-R26 Met -SOD).

To distinguish wild-type MCF-10A cells from the Raf ER-MCF-10A cells, we geneRaf ER-MCF-10Aype MCF-10A cell line that stably expRaf ER-MCF-10A-GFP fusion protein.

In order to introduce more widely available tools to probe these issues in the SNCA field, MJFF has generated: a wild-type human SNCA-expressing strain (a much-needed control in the research field), an mThy-1 SNCA stoain (toverexpressSNCANCA protein in the brain, including in the nigrostriatal pathway), and a knockout mouse (see Tables 1 and 2) (Fleming et al., 2006; Rockenstein et al., 2002).

Therefore, there is no functional advantage to the organism incurred by generating a wild type recombinant mtDNA under such a condition.

The vector pRS316 was used to generate a wild type GCN5 (pRS316+GCN5-WT pRS316+GCN5-WTe andno acid substheution of Glu to Gln (E173Q; pRsingleGCN5-E173Q) representing functionaminon5 acida catalytically dead mutant of GCN5, resubstitution

Furthermore, it is imperative to generate a wild-type virus in parallel with the TC tagged virus in the presence of the biarsenical dye in order to evaluate the specificity of the labeling and the potential packaging of non-specifically labeled cellular proteins.

To obtain the target values AHD, we generate a wild-type network and a mutated network with a single mutated node.

Mice heterozygous for the ApcMin allele were identified by PCR as described previously that generates a wild-type (123 bp) and mutant ApcMin (144 bp) band [ 22].

The function of both genes is also required in xanthophores; mutant xanthophores, when confronted with wild-type melanophores in the chimeras, do not generate a wild-type pattern but spot or disperse melanophores in large dense iridophore regions.