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All three generated a single band of DNA.
PCR using PGB02 BAC DNA and primers placed 1,112 bp and 993 bp on either side of the gap generated a single band of approximately 2.2 kbp.
All real-time PCR assays generated a single band of the expected size, and therefore accurately represented the expression of the queried gene.
The former generated a single band in all three lanes (Fig. 4), which corresponds to the known size of Blcap_v1a (2079 bp), the more abundant of the two Blcap transcripts.
All primer sets used in this study produced only one peak in their respective melting curves, and corresponding amplicons detected under our RT-qPCR conditions generated a single band of expected size on 2% agarose gels (data not shown).
A multiplex agarose gel based RT-PCR that targeted VP1 of O, A, C, and Asia 1 (primers P33, P38, P87 92, P40, P74 77) (7 ) generated a single band corresponding to the size expected (702 bp) for serotype A for all 5 samples (data not shown).
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The wild-type Arg194Trp allele generates a single band representing the entire 485 bp fragment and the variant 194Trp allele generates 396 and 89 bp fragments.
Haplotypes without the deletion generate a single band of 1917 bp in size, which contains amplicons from Saha-UA, UB and UC genes.
Patients with SCCA score ⩽1 showed values of MIB-1-LI significantly lower than those obtained in patients with serpin score ⩾2 (mean+s.d.: 2%±2.4 ([range 0.1−6.3%)] vs 7.5%±10.3 ([range 0.2−48%)], P<0.05), as shown in Figure 3. Squamous cell carcinoma antigen mRNAs were detectable by heminested PCR in 14 out of 18 tumour samples, generating a single band at the expected size of 945 bp.
In marked contrast, chemokines incubated with trypsin and particles (Fig. 10, Lane 3, 6, 9) generated a single species band that was not fragmented, suggesting that particles successfully preserved biomarkers from proteolysis.
In marked contrast, PDGF incubated with trypsin and core-shell particles generated a single species band that was not diminished in staining intensity and was not fragmented, suggesting that particles successfully preserved PDGF from proteolysis (Fig. 9A and Fig. 9B Lane 4).
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