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The software generated a present (P), marginal (M), or absent (A) call for each transcript based on the P value.
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A combined analysis of their sequences along with the sequences used in this study generated a tree presented in Figure 2. Combined analysis did not show any variation from the individual analysis of the two datasets.
To confirm this finding, we generated a Qa1b SCT presenting the Qdm peptide, and three elongated forms: Qa1SCT-CD2, QandCT-CD22 and Qa1-CD4, analogous to the three elongated forms of KbOVA SCT 2. These constructs were expressed in CHO cells and the transfectants were FACS-sorted to give very similar levels of cells surface expression of the Qa1b molecule (data not shown).
However, it is alternatively possible that the DQA*21 allele has acquired a new, unknown function as the frame-shift mutation present in the allele generated a stop codon present at the very end of the gene, thus only truncating the protein by four amino acid residues (Additional file 2; Figure 2).
First, a set of candidate CNV loci is generated (a schema is presented in Figure 1).
This out of frame translation generated a 201aa-long sequence presenting several neighbouring clusters of arginine residues, which somehow resembled basic type nuclear localization signals.
A 6.678 bp product was generated from a present WT -allele.
The probability of an alternative generating a net present value greater than the upper bound value (one Million Rials) is illustrated by a green region; thus, good.
The probability of a risky alternative generating a net present value less than the lower bound value (0) is illustrated by a red region on a bar graph; thus, bad.
Therefore, for almost any realistic oil price scenario, the 20-year cyclic nitrogen injection project will generate a net present value greater than zero within the operational ranges studied.
The MESP is the minimum price that ethanol must sell for in order to generate a net present value (NPV) of zero for a 10% internal rate of return (IRR).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com