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Following Tonikian et al., we generated a position weight matrix (PWM) for every single-mutant PDZ variant based on its set of binding peptides, adding no pseudo-counts and correcting for codon bias by dividing observed amino acid frequencies by their expected frequencies in the NNK codon set [7].
Second, the effect of muscle history of the reference arm, such as muscle fatigue and conditioning might have generated a position matching error because of the initial status of the muscle spindles.
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The collection of sites was then used to generate a position frequency scoring matrix.
Nr2e1 binding sites containing sequences extracted from the literature and used to generate a position weight matrix.
The thin film characterization data is used to generate a position axis from the piezo-drive voltage and to correct for variations in the two-photon response at different positions of the temporal focus.
We generate a position-specific score matrix (PSSM) for a template and a position-specific frequency matrix (PSFM) for a target using PSI-BLAST (Altschul et al., 1997) with five iterations and an E-value of 0.001.
I-TASSER then uses this BLAST result to generate a position-specific scoring matrix (PSSM or profile) using sequences with an E value lower than the threshold (0.005 is the default).
The L. bicolor urate oxidase protein sequence (EC 1.7.3.3, Protein ID 190858) generates a position-specific scoring matrix (PSSM) alignment to the conserved domain for urate oxidase (d00445) with and E-value of 2.03e-61.
In Resch [28], mobile users generate a false position and location of their true position and send it to the server.
To identify informative SNPs, we first generated a list of positions at which we expect bi-allelic SNPs to be segregating among the eight founder haplotypes contributing to a given population; all other sites were ignored.
The setpoints are generated by a position controller which is designed based on a dynamical model of the LIM.
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