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In this report, we generated a modified high-fidelity version of base editor 2 (HF2-BE2), and investigated its base editing efficacy in mouse embryos.
To demonstrate the feasibility of this approach in elucidating the cause of outbreaks from hitherto novel or unknown viruses, we generated a modified NT database from which all sequences corresponding to the Orthomyxoviridae family had been removed (Fig. 8A).
To enable production of GrB with an N-terminus identical to that of the natural protein, we generated a modified pPIC9-MBP-fur-GrB construct which carries a furin recognition motif (fur) lacking the C-terminal serine of furS.
To test this we generated a modified vector based on pMP8.
Therefore, we generated a modified version of QUICKI, the novel IRAR index by taking account into adiponectin and resistin levels.
We generated a modified fluorescence resonance energy transfer (FRET) based PKA activity sensor using the A-kinase activity reporter (AKAR2) backbone (Zhang et al., 2005).
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Comparing with the direct Langevin algorithm, the new method generates a modified Markov chain by incorporating the coarse-scale information of the problem.
As an example of the notation's utility, it is used to generate a modified earned value approach that weights quantities according to their position in a project's timeline.
To evaluate the physiological function(s) of PDZ-RhoGEF, we generated a genetically modified mouse line bearing a conditionally targeted PDZ-RhoGEF allele, Arhgef11.
In light of our previous work we asked whether WNK regulated blood pressure via SPAK and generated a genetically modified mouse in which WNK can no longer switch SPAK on.
Final models were generated using a modified protocol from Lindert et al. Twenty thousand decoys were generated using a simultaneously combined BCL EM-Fold and the memBCL EM-Foldn protocol in BCL::MP-fold.
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