Sentence examples for generated a defined from inspiring English sources

Given the importance of this outer membrane lipoprotein (designated FopC) in F. tularensis virulence and pathogenicity, we generated a defined F. novicida FTN_0444 (homolog to FTT0918, 99% identity in aa sequence) mutant, designated KKF332, and characterized the contribution of this protein to the virulence of this microorganism.

Cell viability was determined by WST-1 assay, cell growth using BrdU uptake, cell migration by chemotaxis assay and wound closure by CytoSelect ™Wound Healing Assay Kit which generated a defined "wound field".

Within these assemblies multiple α-helices supercoil around one another to generate a defined structure which can act as a matrix in which to engineer metal binding sites.

Further we used a kit, which overcomes the disadvantage of common scratch wound assays lacking a defined wound area by providing proprietary treated inserts that generate a defined wound field.

Another approach to generating a defined SP substrate was irradiation of an oligonucleotide containing a TT sequence in a dry film containing picolinic acid, followed by HPLC purification and enzymatic excision of the damaged oligonucleotide.

All proteins from a sample of interest are usually extracted and digested with one or several proteases (typically trypsin alone or in combination with Lys-C [ 1]) to generate a defined set of peptides.

Programming of the microcontroller is integrated into the SPLASSH control software such that it sets up the microcontroller to generate a defined strobe order at the start of every new image acquisition sequence.

To investigate the effects of these parameters and specifically to test the robustness of the results with variation in the parameters, we generated an artificial dataset in which motifs with defined sequence content and positioning were inserted into sequences generated from a defined dinucleotide background.

The initial population is randomly generated inside a defined search space to ensure large consideration of various potential solutions.

The resulting CD36+ cells were generated with a defined combination of growth factors [7].

As the input cell suspension is generated in a defined (X-Vivo10TM) medium formulation, there is no additional requirement for xenobiotics or undefined components.