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All unfixed control samples generated a coverage of 97%, the theoretical maximum by design.
Isolate O/UKG/1450/2001, which exhibited the highest viral load and total numbers of reads, generated a coverage depth >22 across 99.72% of the genome.
Of the 75 samples with less than optimal DNA input, 32 samples still generated the maximum possible coverage of approximately 97%, and a further 20 samples generated a coverage of >95% at a minimum read depth of 100x.
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This generates a coverage spectrum (or bin spectrum) that embodies the observed number of bins containing various numbers of reads in the sample.
Read pileups were generated for each library and all read pileups (n = 11) were pooled using custom scripts to generate a coverage map for defining the genomic distribution of NATs.
To gain more insight into the genome-wide distribution of NATs, we took advantage of the unprecedented depth of our dataset (~600 million mapped reads, Figure 2) and combined the mapping results of 11 libraries to generate a coverage map.
Subsequently, we identified all enriched regions with a peak-height ≥10 and generated a thresholded coverage WIG file for KAP1, using FindPeaks.
Our approach using RNA that was extracted and mixed from four different tissues generated a high coverage transcriptome while controlling, at least in part, for tissue specificity.
The SLIV program in Honolulu County generated a vaccination coverage rate comparable with that of the other 3 less populous counties, each with 60,000 172,000 residents.
Joint community sequencing efforts have generated a high-coverage, still-unpublished reference genome for this organism, which was derived from sperm DNA of FL2 line male worms (Simakov et al. 2013; http://4dx.embl.de/platy/).de/platy/
This topic has generated a variety of coverage, presumably in part because the negotiation is with a book publisher instead of a supplier of a different type of product.
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