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To address this, we generated a caps trn double null mutation, capsDel1 trn28.4, by using P-element induced male recombination [21] to simultaneously delete caps and recombine the new mutation onto the existing trn28.4 null allele (see Figure 3B and Materials and Methods for details).
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Interestingly, they find that at lower temperatures (approx. 20°C) the actin network breaks symmetry and generates a cap, which does not happen at higher temperatures.
To assess whether this sequence is important for the interaction between Cap-H2 and Mrg15, we used site-directed mutagenesis to generate a Cap-H2 Mrg-binding motif mutant (Cap-H2-MBM-EGFP).
While a national system of carbon emission cap and trade does not exist, unilateral action by some states to mandate industry caps has generated a demand for offsets.
We don't rely on our ability to lose money to generate a market cap.
NaGIR1 is a group I-like ribozyme with an evolved biological role in intron NaHEG expression [ 29, 30], likely to generate a lariat cap-structure at the 5' end of the Naegleria homing endonuclease messenger [ 30].
Calling in regulators on both sides of the Atlantic generated a storm of negative publicity, capped by the resignation of HP's chairman and two board directors last week after a shareholder revolt.
The President's 2010 Budget Outline specifically dedicated $15 billion per year in new revenue generated by a cap and trade program to this purpose.
Shares of CPC of America have doubled from early October to a recent $20, generating a $168 million market cap.
Right in Cuban's home city, shares of Manchester Inc. have risen 222% in the past 21 months to a recent $6.35, generating a $210 million market cap on paper.
The solar wind interaction with the Earth's magnetic field generates a cross polar cap potential difference in the range 30 60 kV (Papitashvili et al., 1999; Rycroft et al., 2000), and which, during enhanced solar activity, may exceed 100 kV and expand equatorwards (Boyle et al., 1997; Rycroft et al., 2000; Siingh et al., 2011a; Williams and Mareev, 2013).
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