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To directly investigate the efficiency with which caspase-2 cleaves this sequence in the context of a protein, we generated a Bid mutant in which the natural cleavage site LQTD↓G was replaced with the DTTD↓G sequence.
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But the new asking price had generated a bidding war.
Each mover sets its price for various aspects of a move, and when Unpakt gets a detailed inventory from a customer through its Web site, it automatically generates a bid from each of its movers.
Each participator generates a bid (a quantity and a price) for every bid period (e.g., 5 minutes), and communicates with the aggregating entity.
Julian Hutter, a broker with Prudential Douglas Elliman, cut $50,000 off the price on an Upper East Side studio originally listed just under $450,000, in the hopes of generating a bidding war and a quick sale.
By this point, Say Anything's releases had generated "a major bidding war".
In the last week, Mr. Jackson, 44, has generated a frenzy of bidding and counterbidding for Jackson-related programming among the four major television networks, all seeking an edge in the special ratings period known as the sweeps month.
In its letter, Southeastern Asset Management said: We acknowledge that today's low market price is far below the company's net asset value per share and would not encourage any action that would generate a lowball bid versus this N.A.V.
Geoff Holdcroft, cabinet member at the Conservative-controlled council, said: "If cabinet backs the proposal to widen our net to try and attract someone ready and capable of taking on the Spa, then I look forward to Lisa and her team uniting the expertise and business acumen in Felixstowe to generate a new bid".
To further confirm Bid phosphorylation by JNK and to get insight into the cellular role of this process, we have generated a specific antibody recognizing Bid phosphorylated at Thr59.
To confirm the MS data, we generated a phosphospecific antibody to Bid S66, which detected the slower migrating form of mBidYFP-WT.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com