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(4) Push results to the laboratory director for final decisions on what variants to report and their interpretations, and generate variant tables for final reports.
As an example, we describe the RIG workflow using Sorghum bicolor sequence data and show that it readily interrelates reduced representation and WGS data to generate variant calls.
To demonstrate the utility of these histidine analogues, we substituted His66 in the blue fluorescent protein (BFP) with these UAAs to generate variant BFPs with altered spectral properties.
DOI: http://dx.doi.org/10.7554/eLife.03110.006 Subclones derived from cells showing altered fluorescence continued to generate variant cell populations during expansion.
The mutational spectrum of Qβ replicase compiled on the RGS was directly compared with three DNA-based protocols traditionally used to randomly mutate DNA and generate variant libraries.
Cancer is an evolutionary process where differences in microenvironmental selection pressures, iatrogenic intervention and dynamic cellular changes over time constantly generate variant subpopulations among systemically spread cancer cells (Greaves & Maley, 2012; Klein, 2013).
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But these are hard points to prove because researchers can't generate variants or observe naturally occurring ones.
Numerous methods, including random mutagenesis, site-directed mutagenesis and DNA shuffling, have been widely used to generate variants of existing enzymes.
We engineered the Fc domain of an anti-CD19 antibody to generate variants with up to ∼430-fold greater affinity to FcγRIIb.
Evolution is just a consequence of the imperfect replication of informative polymers that generate variants upon which drift or natural selection act.
If the last word is a letter or letter followed by a digit, generate variants where the preceding space is replaced by a hyphen, e.g. 'Bisphenol A' will also give 'Bisphenol-A'.
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