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Protein-coding genes could generate transcripts from lncRNAs genes.

The reactions were incubated for 60 min at 37°C and then stopped by the addition of 15 µl of loading buffer followed by heating to 75°C for 10 min. The in vitro generated transcripts from the linear templates, which contained the cloned PhilD or PhilD*, the vector promoter (Pν), and the supercoiled pUA1111 vector, were separated in 6% dPAGE, visualized, and quantified as described [65].

The BLCAP/ Blcap locus generates transcripts from distinct promoters downstream (BLCAP_V1/ Blcandv1) and upstream (BLCAP_V2/ Blcap_v2) of NNAT/ Nnat (Fig.  1).

The generated transcripts from four developmental stages, infective L3, serum stimulated L3, adult male and adult female, covered 93% of the A. caninum transcriptome.

The contigs unique to the study of Woodard et al. [ 32] may derive from rare transcripts, or from differences in origin of samples as Woodard et al. [ 32] generated transcripts from only brains and abdomens of workers while we used whole bodies of several stages.

A major process generating transcript diversity from a single gene is through alternative splicing (AS), whereby amongst other mechanisms variable inclusion of exons and altered exon boundaries can be used to produce different transcripts.

Like APA, AS is a regulatory mechanism to generate multiple transcripts from one single gene.

Not only APA can produce multiple isoforms from the same genes, but alternative splicing also can generate different transcripts from the same genes.

However, in the silkworm (B. mori), alternative splicing seemed to generate two transcripts from a single copy of the SCP-x/SCP-2 gene [ 27].

Alternative splicing (AS) generates multiple transcripts from the same gene by differential splicing of introns, thereby increasing transcriptome and proteome diversity [1].

Splicing generates multiple transcripts from one gene.