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During a sequencing run, the Polonator captures and analyzes 5.7 million such images to generate the sequence of each fragment.
This class has a main method and is used to generate the sequence of images for the experiment.
The generation probability (Pgen) of a nucleotide or amino acid CDR3 is the sum of all model probabilities of V(D J recombination events that generate the sequence.
We adopt the Halpern iterative scheme to generate the sequence.
The Gibbs sampler (Geman and Geman 1984) is used to generate the sequence of iterations.
The number of cycles required to generate the sequence is sum_{i=0}^{n - 2} leftlceil frac{n - i- 1}{2m - 1} rightrceil.
Similar(32)
TruSeq® DNA PCR-Free Sample Preparation Kit (Illumina, USA) were used to generate the sequencing libraries.
For each sequence, dissectHMMER will be applied to generate the sequence-to-domain alignment hits and the associated error-adjusted domain coverage mesasures (for both ratio and fold-critical E-value measures; see Eq. 5).
We used rose[ 24] to generate the sequences and r8s[ 25] to generate the trees.
The standard pipeline to generate the sequencing output files are described in the Supplemental Information.
In single-end reading, the sequencer reads a fragment from only one end to the other, generating the sequence of base pairs.
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