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Our approach can be used to generate templates from any DNA source for next-generation sequencing.
That was used to generate templates that enabled the panels to be cut in steel and etched.
The number of comparisons also varies because a few images do not generate templates, so some databases had fewer templates for comparison than other databases.
In this paper, we present a new method based on particle swarm optimization (PSO) to generate templates for frontal face localization in real time.
We construct triphone context-dependent phone templates to preserve the time dynamic information of phone units and use phonetic decision trees to generate templates of tied triphone units, which improves the reliability of triphone templates and covers unseen triphones by some triphone clusters.
Further recombination/ligation events between such simple RNA molecules could generate templates encoding sufficiently complex protein products (primordial mRNAs).
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cDNA was generated from 1 ug of RNA using Omniscript reverse transcriptase (Qiagen) and oligo-dT primers to generate template for GAPDH quantification and an ORF50-specific reverse primer (5' GGAGAGGGACTACTGTTGG 3') to generate template for ORF50 mRNA quantification.
Primer set MT2/MT19 (15424-102) was used to generate template for nested amplification with D1 primers (15898 16525).
Primers to generate template for probes: 5′-AGTTCATTGGGACCATCCTGG-3′(F), 5′-CGTGCACATAACAGACTTGGC-3′(R).
A diluted library (26 pM) was used to generate template positive Ion Sphere™ Particles (ISPs) containing clonally amplified DNA.
Previous studies have shown that these extraction protocols generate template DNA from double-stranded DNA viruses that is suitable for amplification by real-time PCR [ 12, 27].
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