Sentence examples for generate substitution from inspiring English sources

Since an appealing hypothesis would be that these peptides are generated by the virus to act as antimicrobial agents that favor viral infectivity in their fish host, we decided to test this possibility by selecting a master peptide and using it to generate substitution variants that may enhance their antimicrobial potential.

The majority of sarcoglycanopathies are associated with missense mutations that generate substitution of single residues that could lead to a misfolded protein.

The QuikChange II XL Site-Directed Mutagenesis Kit (Stratagene) was used to generate substitution mutants of the human FPGS mitochondrial and cytosolic initiation codons (ATG) from the pGL2628 plasmid using oligonucleotides QC19934F/QC19934R and QC20060F/QC20060R, respectively (Table 1).

Many mutations in this category generate substitutions of highly conserved glycine residues, including the following: Gly67 in the chromogenic tripeptide; Gly31, Gly33, and Gly35 in the second β-strand; and Gly20, Gly91, and Gly127 in the lids of the β-barrel scaffold.

Marx (2001) reviews various studies also generating substitution and displacement costs between 20% and 50%.

To test this hypothesis, we generated substitution variants R1838A and R1838C of mouse Shroom3 SD2 and tested their ability to bind to the Shroom-Binding domain (SBD) of human (h) Rock1 using in vitro binding assays.

By generating substitutions at Lys180 that either abolished or mimicked acetylation, we provide in vitro and in vivo evidence that further implicate Lys180 as a critical residue for RcsB-dependent repression of the flhDC genes.

The specific mutations generating substitutions of the aspartic acid and valine residues at positions 710 and 711 with alanine and isoleucine (D710A and V711I) in the RT motif A of hTERT result in the ablation of authentic telomerase activity and confer dominant-negative properties against the authentic telomerase activity and canonical function of TERT (DN-hTERT) [76], [78].

The lack of 3′-5′ exonucleactivityvity explains its low fidelity, generating substitutions at a frequency 10too 100 times higher than observed with other family A polymerases [ 13].

Finally, we quantified the overall ratio at which recombination and mutation events (r/m) have generated substitutions in the strains sequenced here.

To estimate the overall ratio at which recombination and mutation events (r/m) had generated substitutions, ClonalFrame [ 98] was run on genes previously used in multilocus sequence typing of Lactobacillus casei (fusA, ileS, leuS, pyrG, recA and recG) [ 99], and Bifidobacterium spp. (fusA, ileS, gyrB, rplB and rpoB).