Although we did not generate stable transformants with Rpi-pta1, similar results are expected with Rpi-pta1.
The results showed that higher pressures could generate stable bending motions with smaller errors along the z-axis.
Experimental results show that the proposed method can generate stable, smooth, orderly, regular and elegant crowd formation animations.
Therefore, taken together the results clearly illustrate that the Bxb1- att system performs genomic excision removing unwanted DNA to generate stable recombinase-free Arabidopsis transgenic plants.
In this study, we utilized temporally controllable reagents—small-molecule ERK inhibitors in planarians and heat-shock mediated overexpression of transgenes in fish to generate stable missing-tissue contexts, in which the regeneration-inducing capacity of wound signals could be tested.
Multi-city studies are believed to generate more stable results which are less prone to biases that may affect small studies in individual centers [ 6].
To reduce the solution space and to generate more stable results, we used the preselected set of 387 genes as input for the GALGO program.
Based on these results, we generated stable RK13 clones expressing WT PrP (WT-10), S1 PrP (S1 29), S3 PrP (S3 27) and S3.F88W PrP (F88W-5).
Next, to confirm the results of NAC1-knockdown experiments, we generated stable NAC1-expressing cells from ES2 cells, which have low endogenous NAC1 expression.
The results show that the TiB2 heater is capable of generating stable temperatures up to 2000 K.
Therefore, taken together the results clearly establish that the phiC31 system performs genomic excision, generating stable transgenic recombinase-free Arabidopsis plants with unwanted DNA removed.
