Sentence examples for generate spheres from inspiring English sources
CD44v3highALDH1high cells dissociated from spheres were counted by hemacytometer and replated to generate spheres of next generation.
That is, an initial number of cells are seeded (e.g., 2.5×105), which generate spheres and produce a total cell count for the flask (e.g., 1×106), a portion of which are harvested (e.g., 25% are taken) and used to seed another flask for passage.
A property of stem cells is the ability to generate spheres.
In the present study, we show that the SP of PDAC is enriched in cells that generate spheres.
The ALDHhigh - and ALDHlow -sorted fractions were able to demonstrate phenotypic heterogeneity and generate spheres, the latter being less efficient, and both showed an association with CD44.
In addition, it has been demonstrated that only NB stage-IV-derived cells generate spheres, but that the MYCN expression status is not related to the sphere formation.
In the present study, we used a panel of established human colon cancer cell lines to generate sphere cultures.
We introduce a new geometric method to generate sphere packings with restricted overlap values.
(d) In neurosphere suspension culture condition, primary HPCa202N basal cells generated spheres more efficiently than luminal cells.
When the concentration was fixed at 0.1 M, the average diameter of the generated spheres is ~93 nm (see Fig. 1f), with a size distribution of standard deviation of ~11%.
In particular, the localization of binding site(s) is composed of three related operations: 1) generating the molecular surface of the receptor using the DMS program; 2) generating spheres surrounding the receptor using the Sphgen program; and 3) selecting a subset of spheres to represent the binding site(s) using the Sphere_selector program.
