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You have to generate some sense of bigness on your own; that's an insufferable activity".
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Time will tell whether "Past Life" has the same problem as "Medium," in which the continuing doubts of Allison's husband and colleagues — apparently necessary to generate some dramatic tension — make no sense after six seasons of her being right every time.
These sense and anti-sense DNA templates were used to generate sense and anti-sense transcripts using BLOCK-iT RNA TOPO transcription kit (Invitrogen, USA).
To generate sense and anti sense RNA, pGEM-T: NtCesA was linearized using NcoI or SalI and in vitro transcription was performed (RiboMAX, Promega) with SP6 and T7 RNA polymerases.
Vector was linearized with the appropriate enzymes and transcribed with digoxigenin UTP (Roche, Mississauga, Ontario) to generate sense and anti-sense riboprobes as we described previously [19].
Full-length Kirrel1, 2, and 3 cDNAs were used to generate sense and anti-sense probes.
T3- and T7-mediated in vitro transcription was performed to generate sense and anti-sense RNA probes.
The PCR product was purified (Invitrogen PCR cleanup kit) and used in combination with the RiboMAX Large Scale RNA Production System (Promega) to generate sense and anti-sense RNA strands that were subsequently annealed together to generate the final dsRNA product.
cDNA inserts were prepared from breast cancer cDNA libraries, subcloned in pBluescript II (MMP-2 and MMP-14) and pBS KS- (TIMP-2) vectors and were used as templates for in vitro transcription to generate sense and anti-sense probes.
The T7 promoter from the TA cloning vector was used to generate both anti-sense and sense probes.
Such proceedings generate no sense of detachment.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com