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These sensors generally generate sensing data in fixed rates, so their transmission schedules can be deterministically listed.
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After cleanup, 10 µg cRNA was used to generate sense cDNA.
Vector was linearized with the appropriate enzymes and transcribed with digoxigenin UTP (Roche, Mississauga, Ontario) to generate sense and anti-sense riboprobes as we described previously [19].
These arrays feature a new chip design with no mismatch probes and a radically new random primed protocol to generate sense DNA targets along the entire length of the transcript.
The strategy for LIC-based cloning of inverted repeat constructs is outlined in Figure 2. In the first round of PCR the target sequence is amplified with two pairs of primers to generate sense and antisense products.
The amplicon was cloned and used to generate sense and antisense riboprobes for northern blot analysis and fluorescent in situ hybridization (FISH) experiments using T7 and T3 RNA polymerases, respectively.
Full-length Kirrel1, 2, and 3 cDNAs were used to generate sense and anti-sense probes.
and was used to generate sense and antisense DIG (digoxigenin -labelled riboprobes.
T3- and T7-mediated in vitro transcription was performed to generate sense and anti-sense RNA probes.
These sense and anti-sense DNA templates were used to generate sense and anti-sense transcripts using BLOCK-iT RNA TOPO transcription kit (Invitrogen, USA).
The ClToxB was cloned into a dual promoter silencing vector pSilent-Dual 1 to generate sense and antisense ClToxB RNA pools.
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