A voltage controlled oscillator, which has multiphase structure, is combined with the multi-layer delay chain to generate quadruple phases, and thus expands the overall resolution.
We generated quadruple mutant of rd26 anac019 anac055 anac102.
To understand this hormone co-regulation, we generated quadruple mutants for the HLS1 gene family and also further characterized their role as regulatory hub signal integrators.
Having generated quadruple mutants with combination of loss-of-function mutations in all group I members of single-repeat R3 MYB genes, including CPC, ETC1, TCL1 and ETC3, we sought to generate higher order mutants.
To generate ppcsp quadruple deletion mutants, the PpCSP1-deletion construct was introduced into wild-type Physcomitrella to generate ppcsp1 lines.
A cascaded combination of two MZMs was used to generate frequency quadrupling [16], sextupling [8, 17] and octupling [18].
To generate the quadruple mutant chy1chy2lut2lut5, the homozygous triple mutant chy1chy2lut2 was crossed with the homozygous single mutant lut5 [ 12].
Genetic crosses between dcl2/3/4 and above-mentioned single mutants were performed to generate the quadruple mutants: rdr2-1 dcl2-5 dcl3-1 dcl4-2, nrpe1-11dcl2-5 dcl3-1 dcl4-2, nrpe1-11 dcl2-5 dcl3-1 dcl4-2 and ago4-7 dcl2-5 dcl3-1 dcl4-2.
To generate the quadruple mutant chy1chy2lut2lut5, the triple mutant chy1chy2lut2 and the single mutant lut5 were crossed, and F1 seeds were grown and self-fertilized to obtain the F2 generation.
In addition, the amino group in Lys is generally more reactive than the guanidino group of Arg because of its lower p Ka; thus, we chose to replace His-18 in the human peptide with Arg to generate the quadruple mutant, H18R/A25P/S28P/S29P IAPP, denoted QM.
To this end, we generated one quadruple-point mutant with four threonine residues mutated to alanine (A) (referred to as Ezh2-M 4A), and one triple-point mutant with a T372 residue remaining intact (referred to as Ezh2-M 3A372T).
