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Basal phosphorylation of sarcoplasmic reticulum (SR) Ca2 + proteins is high in sinoatrial nodal cells (SANC), which generate partially synchronized, spontaneous, rhythmic, diastolic local Ca2 + releases (LCRs), but low in ventricular myocytes (VM), which exhibit rare diastolic, stochastic SR-generated Ca2 + sparks.

These techniques use theoretical wind spectra as argument of stochastic functions that generate partially correlated data series which once fed into a numerical integration procedure gives synthetic dynamic response histories.

To this aim, we employed three pairs of partially complementary DNA oligonucleotides, to generate partially dsDNA substrates with either 5'- or a 3'- protruding ss ends, or both (Fig. 6 A).

Combinations of consensus primers were used to generate partially overlapping amplicons that covered the complete genome.

The assay also allows detection of mutations that generate partially functional proteins as exemplified by ppcesa5R453K.

Potentially, the ssDNA fragments generated by these nucleases may re-anneal and experience further processing to generate partially duplex molecules of defined size prior to integration by Cas1.

Rabbit sera raised against them were able to generate partially-neutralizing antibodies, which delayed mice mortality in neutralization assays against Naja haje, Dendrospis polylepis and Ophiophagus hannah venoms.

V00654) [36] to generate partially-overlapping polymerase chain reaction (PCR) amplicons, with an average length of 646 bp, spanning the complete sequence of the aurochs mtDNA genome.

To generate partially-purified, enriched unlinkase activity fractions via cation-and-anion exchange chromatography (Fraction CA), RSW was subjected to cation-exchange chromatography (SP Sepharose FF, GE Healthcare), and proteins were eluted from the column using increasing concentrations of NaCl in unlinkase buffer.

To generate partially-purified, enriched unlinkase activity fractions using sucrose gradient fractionation and anion-exchange chromatography (Fraction SA), RSW was initially applied to one of two gradients: 7%–47% sucrose w/w (20 mM Tris pH 7.5, 5 mM MgCl2, 100 mM KCl) or 15 30% sucrose w/w (20 mM Tris pH 7.5, 5 mM MgCl2, 100 mM KCl).

The small droplets generated partially evaporate and cool down until thermal and mechanical equilibrium is reached.