His skill is not in generating nonsense, but knowing how to use it – what to leave, what to weave, what to conflate, what to reincorporate.
All other mutations except two transposon insertions generated stop codons, were deletions, an inversion, or frameshift generated nonsense codons, even those which exhibited only one or two of the three dumpy mutant phenotypes.
Wherever possible, we used CODDLE (Codons Optimized to Discover Deleterious LEsions) [ 25] to identify exons in which ENU has the highest likelihood of generating nonsense mutations.
More importantly, we found that the patterns of alternative splicing in some key circadian genes can be very complex and in most cases generates nonsense isoforms.
Geisel died in 1991, but had he lived long enough to observe current affairs even he might have been surprised at our political and economic system's ability to generate plausible nonsense of its own.
As a control, a 19 nt sequence derived from the Oryza sativa genome (GenBank EF576615.1) was cloned into pBlueU6 to generate pBlueU6-nonsense (NS) plasmid.
In the HAP1 hoc gene the transition C496→T occurs, thereby generating a nonsense mutation Gln166→ orche stop codon which was confirmed to stop incorporation of Hoc into the phage capsid [ 22].
The latter seems to be more likely in our case because the point mutation in codon #249 in exon 7, which was shared by all of the cell systems, should have generated a nonsense gene product, which could not be recognized by the antibody whose antigenic site is the transcriptional transactivation domain within the NH2-terminal region [ 40].
Thank God we'll still have Joe Biden, and with Bill O'Reilly continuing to be a nightly factor, it's not like we'll have to generate all the nonsense ourselves.
