Sentence examples similar to generate minion from inspiring English sources

The ultra-long-read-length sequences generated by MinION have been used for de novo genome assembly and non-reference scaffold building (Quick et al. 2014; Ashton et al. 2015; Jain et al. 2015; Kilianski et al. 2015).

Noteworthy, the alignment-based approach that was used for the calculation of the total mean sequencing error rate revealed that several MinION reads were composed of non-contiguous regions of the YCNV genome, suggesting that either defective viral variants were sequenced or that the MinION generated chimeric sequences.

Sequencing short-DNA-fragment libraries prepared using our protocol with MinION generated ∼500 unique reads after the first 3 min of sequencing and 43,000 87,000 raw reads and 27,000 58,000 2D reads (32 67%) after 4 hr of sequencing.

To determine the optimal tool for data analysis of the increased number of reads obtained with sequencing of short DNA, we compared LAST an alignment program recommended by MAP with two similar programs, Bowtie 2 and Blat (Kent 2002; Frith et al. 2010; Langmead and Salzberg 2012) using a training library generated through a MinION short-DNA-sequencing run (Table S3).

Oxford Nanopore Technologies has developed three scalable instruments, including the MinION, a USB-device portable sequencer able to generate up to 16,000 reads/run with a maximum length of 60 kb and an average throughput of 90 Mb in an 18-hour run.

Table 3 Coverage and identity of consensus mapped and de novo assembled genome sequences of yam chlorotic necrosis virus (YCNV) generated from Illumina and MinION reads.

For mild comic distraction in a movie that is skimpier than most in generating laughter, there is Minion (David Cross), Megamind's loyal assistant, who has a fishbowl head, the body of a robot gorilla and a fanged underbite.

poretools fasta / path/to/fast5/example.fast5 poretools fastq / path/to/fast5/example.fast5 poretools fasta / path/to/fast5/example.fast5 poretools fastq / path/to/fast5/example.fast5 At the time of writing, each MinION run generates individual HDF5/FAST5 files for each sequenced read.

NIPT testing on the MinION nanopore sequencer using the standard protocol took 6 24 hr to generate up to 56,000 2D reads, and 15.6 23.9% of the 2D reads were UA reads (Cheng et al. 2015).

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The Genomic DNA Sequencing Kit, SQK-MAP-002 (Oxford Nanopore Technologies Ltd , Oxford UK), was used to generate MinION® sequencing libraries.