Sentence examples for generate gateway from inspiring English sources

The amplicons were cloned into pENTR/D-TOPO (Invitrogen) to generate gateway entry constructs, which were fully sequenced to ensure sequence integrity.

PCR fragments were then cloned in either, Grand Island, NY P221 (Invitrogen) or pENTR-D/TOPO (Invitrogen) to generate gateway (Invitrogen) entry clones.

Actin-CB and PCNA-CB were cloned into pDONR221 (Invitrogen) to generate Gateway-compatible Middle Entry (pME) constructs.

The two fragments were ligated into pCR8/GW/TOPO (Invitrogen) vector digested by EcoRI to generate a gateway compatible dCas9VP48 coding plasmid.

The keys, members of the one-way key chain, are generated during gateway's initialization and are stored in its memory.

Reporter constructs were generated by Gateway recombination as described above.

The mRFP-containing lentiviral vectors were generated by gateway recombination between the pDONR-221 mRFP entry vector and the pLenti6/V5-DEST destination vector.

Command Node (e.g., sink or Base Station) designates for each cluster a number of key generating gateways (e.g., cluster head), so SHELL is more resilient against gateway compromise.

The 35S NTAP AtCTF7∆B construct was generated using Gateway-compatible binary vectors containing the NTAP tag ((NTAPi) [ 30]; a gift from Dr. Qinn Li lab, Miami Univeristy).

The aim of this research was to identify if it were possible to generate a distinct biological gateway for the production of PAA.

We evaluate the running time taken by our proposed protection gateway to generate the k-anonymity (k = 6), entropy l-diversity (l = 6), recursive (c, l -diversity (4, 6) and t-cl -diversity= 0.15 & 0.2) anonymous data tables for varied sizes of quasi-identifiers and are reported in Fig. 10.