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The resultant PCR product was cloned into pDONR221 (Invitrogen) using BP Clonase II enzyme mix (Invitrogen) to generate entry clones.
The generated amplicons were recombinationally cloned into the pDONR223 Gateway vector to generate Entry clones [ 41].
The chimeras were cloned in pENTR-D/TOPO to generate entry clones.
PCR fragments were cloned directionally after a BP reaction (Invitrogen) into the pDONR vector (Invitrogen) to generate entry vectors.
The verified coding region fragment of SKS13 was transferred into donor vector pDONR207 using the Gateway® BP Clonase™ II enzyme mix (Invitrogen) to generate entry vector pDONR207:: SKS13.
Moss α−tubulin coding sequence was amplified from pAct-GFP-TUA1 (31) with primers (P7 & P8) containing attB5 and attB2 sites and cloned into pDONR-P5P2 to generate entry clone α-tubulin-L5L2.
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Our goal is to extend the work of Chen et al. with respect to the following: Based on their semantic mapping, we develop a more generalized approach for automatically generating entry forms in legacy EHR systems from archetypes.
Aside from generating entry and exit signals, the most productive trading signals that you receive are bullish or bearish divergences.
Simulation results demonstrate that the generated entry trajectories can avoid no-fly zone with a large cross-range and achieve the terminal conditions while satisfying all the complex path constraints.
NS assisted with cell tracking, while SB cloned PMMs into entry vectors, and SW supervised SB and generated entry vector cDNA libraries.
In the case of the human β-globin gene, co-transcriptional cleavage of terminator transcripts generates entry sites for Xrn2 (West et al, 2004).
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