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Stem cells continuously generate differentiating daughter cells and are essential for tissue homeostasis and development.
Therefore, at the next anagen hair follicle stage, the MSCs become activated and generate differentiating, transit-amplifying daughters.
As we only detect one large Ase-negative type II Nb, and the extra cells express the INP marker Ase, we conclude that barc is required for INPs to generate differentiating neurons.
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The ability to generate, differentiate, and profile adult stem cells provides a powerful but underutilized opportunity to investigate the molecular basis for trait differences between species within specific cell types and in a controlled environment.
Cells grown in serum and LIF can differentiate: they spontaneously generate differentiated cells in culture because they are heterogeneous with respect to the expression of a variety of pluripotency-associated genes, and hence are heterogeneous in their immediate differentiation potential.
The constructs were generated by differentiating 3T3-L1 preadiponytes on 3-D polymeric scaffolds for either 9, 21, or 35 days in vitro.
TAM-like properties of CD206high, CD204high, IL-10high, TGF-βhigh, IL-6low, IL-12low, VEGFhigh, and MMP-9high and promotion of tumor cell invasion were more pronounced in M-2-polarized THP-1 macrophages generated by differentiating THP-1 cells with PMA and subsequently polarizing them with Th2 cytokines (IL-4/IL-13).
Adipogel was generated from differentiating murine preadipocytes as described previously.
A spermatogonial stem cell first generates one differentiating spermatogonium and one stem cell.
Human HL-60-neutrophils were generated by differentiating HL-60 with RA.
Primary macrophages were generated by differentiating bone marrow from 6- to 12-week-old C57BL/6 mice for 7 days in Dulbecco's modified Eagle's medium (DMEM) supplemented with 5 ng/ml recombinant M-CSF (R&D systems), 2 mM glutamine, 10% FBS, penicillin and streptomycin.
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