Sentence examples for generate an entry from inspiring English sources

The PCR-amplified DNA fragment was cloned into a pENTR/D-TOPO vector (Invitrogen, Tokyo, Japan) by a BR recombination reaction to generate an entry clone, pENTR-HiDXS.

When you lead an honor to drive out an opposing higher card and generate an entry for your partner, it is called a Deschapelles Coup.

The gateway attB-flanked PCR product was recombined with a donor vector containing the corresponding gateway attP signals in a BP reaction to generate an entry clone.

These data suggest that decapping by Dcp2 can often generate an entry site for Xrn2 to degrade aberrant RNA.

The purified PCR products were cloned by site-specific recombination into the donor vector pDONR221 to generate an entry clone.

Within endo/lysosomal compartments, host endosomal cysteine proteases (cathepsins) cleave the filoviral GP1 protein to generate an entry intermediate comprising an N-terminal GP1 fragment and GP2.

To allow generation of a wide variety of TetR-expressing cell lines, we also generated an Entry vector with the TetR cDNA (pENTR/TetR) and recombined it with the pLenti CMV Blast DEST vector to make pLenti TetR Blast (not shown).

The oligoadenylated 3′ ends that we detect suggest that termination generates an entry site for the TRAMP and exosome complexes.

When West leads the spade king from his hand, trying to generate a dummy entry with the spade jack, North must duck.

The Neomycin resistance gene was amplified by PCR from pcDNA3 vector using the forward primer 5'CandATGATTGAACAAGATGGA3' and reverse primer 5'GandAACtheTCAAGAAGGCGA3', and the resulting PCR product was cloned into the pENTR/D/TOPO plasmid (Invitrogen) to generate a Neomycin entry clone.

The amplified genomic DNA fragment was introduced into pDONR/Zeo plasmid (Invitrogen) to generate a Gateway entry clone and the cloned WNK8 genomic fragment was transferred into the pEarleyGate302 vector [ 30]. the resulting construct was transformed into wnk8-1.