Sentence examples for generate a template from inspiring English sources

To generate a template for use in creating a standard curve, we amplified from cDNA and gel-purified a 53 bp fragment of KRT1, using the following primers: 5' GTGGCAGTTCCAGCGTGA 3' and 5' GCATCTGGTTACTCCGGA 3'.

Use pairwise structure based alignments to generate a template alignment of all PDB sequences.

In regular MLPA the next step would be the ligation of the neighbouring probes to generate a template for PCR.

To generate a template for in vitro transcription, an EBNA1 template was designed using the wild type HHV4 EBNas as a reference sequence (NCBI accession YP_401677.1).

The structure of the Y-adapter/template/hairpin-adapter allows the sequencer to generate a template read, a complementary read, and a calibration of these two reads (i.e., a two-dimensional read for dsDNA).

First, to generate a template PPI network with the characteristics of real yeast PPI networks, we used a geometric graph model with gene duplication and mutation (GEO-GD expansion model; Przulj et al., 2010; Supplementary Fig. S3A).

Briefly, RDRP elongates a primer to copy the viral RNA template (plus strand) and the newly synthesized minus strand folds back on itself to generate a template-primer structure, which is elongated by the RDRP to form covalently linked dimeric RNA molecules [ 6, 7].

Further, we have implemented a function that automatically generates a template for the general tag with the following steps: 1. Discriminating variable words from invariable words in the set of tags on the packages to be generalized   2.

The PCR product was amplified using a 5' primer containing a T7 phage promoter sequence (Sigma) and a 3' primer containing a SP6 phage promoter sequence (Sigma), generating a template for transcription of a sense and an antisense probe, respectively.

Regarding the second possibility, it has been proposed that medial rostral PFC is involved in generating a template against which to match observed information in the environment.

Larval skeleton deposition does not require the presence of zooxanthellae, but generates a template for the skeleton of the initial polyp [ 3, 15, 16].