Exact(4)
Since the oligomerization of p53 involves the rate-limiting formation of a primary dimer intermediate, we engineered a covalently linked pair of human p53 tetramerization (p53tet) domains to generate a tandem dimer (p53tetTD) that minimizes the energetic requirements for forming the primary dimer.
The events that generate a tandem duplication of a single-copy gene are less understood.
Thus a single state of order k can generate a tandem repeat with period k, allowing for mismatches between consecutive motif occurrences.
4. In case of a tandem duplication insertion, the sequence segment of length l starting at position p in the test sequence was duplicated and inserted again at position p. In case of an insertion that was not chosen to generate a tandem duplication, a sequence segment of length l was generated by independently drawing each nucleotide from the distribution of base frequencies in the test sequence.
Similar(56)
Also, in complex samples, it is common to have more than one ion species in the same isolation window to be fragmented when generating a tandem mass spectrum.
Since Ret and Rosa26 loci are located only ∼5 megabases apart on mouse chromosome 6, we generated a tandem configuration of Ret CreERT and Rosa Tdt alleles, which are linked during meiosis and became a great genetic advantage for our experiments.
Next, capture DNA would act as primer to initiate a linear RCA reaction and hence generate a long tandem repeated sequences by phi29 DNA polymerase and dNTPs.
In this system, the PP can be circularized after hybridization with the precisely-matched target DNA, while the stem of MB can be also opened by target DNA, resulting in hybridization with the circularized PP to generate a long tandem single-stranded DNA (ssDNA) product.
A re-flattening iterative method was hence developed to generate a precise TanDEM-X DEM in this study.
Then rolling circle amplification (RCA) reaction can be initiated from the mutant DNA that acts as primer and generates a long tandem single-stranded DNA (ssDNA) product.
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