Sentence examples for generate a suspension from inspiring English sources

Organ tissues were weighed and then pulverized to generate a suspension of tissue in TSB.

Yellow-green fluorescent or colourless beads (Polysciences, Inc., Warrington PA, USA; and Bangs Laboratories, Fishers, IN, USA), ranging in sizes from 1 to 8.31 µm in mean diameter, were suspended in DPBS then briefly sonicated to generate a uniform suspension of single beads as previously described [40], [43].

Vandetanib was prepared with 1% polysorbate 80 (Fluka), diluted in sterile water, and milled overnight to generate a uniform suspension.

After washes, the resulting pellet was resuspended in 2-3 ml of 0.5% Triton-X to generate a spore suspension of no greater than 5 ml.

Frozen tumor samples (100 to 200 mg) were disintegrated mechanically and enzymatically to generate a cell suspension as described previously [ 21].

Spleens were removed under sterile conditions and transferred to a petri dish (Becton Dickinson Labware, Franklin Lakes, NJ, USA) to generate a cell suspension that was passed through a 70-μm-pore filter (Becton Dickinson).

Cells were disassociated with accutase to generate a single-cell suspension (1 1 × 10 cells in 20 μl PBS) and this suspension was injected under the kidney capsule.

The suspension was filtered through a 70-μm stainless steel wire mesh to generate a single-cell suspension.

The spleens were passed through a cell strainer (BD Bioscience) to generate a single-cell suspension that contained splenocytes.

Preparation of lymph cells; Male Balb/c mice (6-week-old) were sensitized with 150 μL of 3% OXA-ethanol solution placed onto the skin of the abdomen at day 0. At day 3, mice were fasted and at day 4, the lymph nodes that were removed from OXA-sensitized mice were passed through a cell strainer (BD Bioscience) to generate a single-cell suspension that contained lymph cells.

Thymus and spleen tissue were homogenized and passed through a cell strainer (BD Biosciences) to generate a single-cell suspension.