To determine the amount of purified JFH-1 virions, the pUC18-JFH1 plasmid was used as a standard sample to generate a standard curve ranging from 103 1011 copies/mL.
This stock solution was then further diluted in assay buffer to generate a standard curve ranging from 0 to 15 nmols of PO4, each in a final volume of 200 μL.
Each gel contained a concentration range of purified STAM2 to generate a standard curve and samples from induced cells for each of the overexpression time points used in the STINT-NMR titration experiments.
In each assay, the corresponding cytokine was diluted over the detection range recommended by the manufacturer to generate a standard curve from which sample concentrations (pg/ml) were calculated.
rhSema4A was tested at concentrations ranging from 1 to 1,000 ng/ml to generate a standard curve.
Genomic DNA obtained from a filtered culture of M. bovis BCG was used to generate a standard curve of genomic equivalents for the real-time PCR over a dilution range from 845000 to 20 units per PCR reaction.
The neutral lipid standard triolein (Sigma, China), which ranged from 2.0 to 100.0 μg/mL, was used to generate a standard curve.
To generate a standard curve, mature recombinant OP-1 (Stryker Biotech) was diluted in TBS/Tween at concentrations ranging from 10 ng/ml to 0.01 ng/ml.
Twelve standard assay points were used to generate a standard curve.
The results are used to generate a standard curve.
