Sentence examples for generate a standard for from inspiring English sources

Given this potential variation in the rRNA gene copy number [6], the strategy of pooling different genomic DNAs derived from several cultured isolates of the same species to generate a standard for quantifying environmental samples may affect the results of the qrt-PCR assay [7].

To generate a standard for the RT-qPCR-based quantification, a suspension with known concentration of RoV particles (1.1 × 10 particles/ml) estimated by electron microscopy counting using latex bead standards was first diluted to concentration 10 particles/ml.

With the Gene Ontology and corresponding annotations in hand, the main issue in generating a standard for evaluation is deciding which terms are specific enough to imply functional associations between gene products.

Extracted DNA from Parvovirus B19 Standard (NIBSC, UK) was used for positive control and to generate a standard curve for quantitation.

Either a plasmid DNA or cDNA dilution series was used to generate a standard curve for quantification of transcript levels and for measuring amplification efficiency.

To generate a standard curve for each genotype, tissue was incubated for 20 40 min in nigericin buffer (25 mM HEPES, 105 mM KCl, 1 mM MgCl2, 20 µM nigericin [Invitrogen]) at defined pH points (∼6.5, ∼7.0 and ∼7.5).

Tenfold dilutions of a counted number of RH tachyzoites and cultured swine kidney cells (SK-6) were used to generate a standard line for the quantification of T. gondii parasite DNA and one for the cells, respectively.

Purified HOBB was dissolved in DMF and diluted with ethyl acetate to generate a standard curve for HPLC, LC MS, and GC MS analysis.

Our recently introduced structure preparation tool SPORES [1] used a rule based method to generate a standard protonation for each ligand molecule and afterwards generated different protonation states in a combinatorial way by adding and removing single hydrogen atoms belonging to predefined functional groups.

Serial dilutions of synthetic HIV-1 RNA were used to generate a standard curve for each assay.

Serial dilutions of total extract DNA (1/10,000, 1/1000, 1/100, and 1/10) were used to generate a standard curve for each primer and each reaction.