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The normalized projection signals from each kind were then averaged to generate a projection voxel map for each injection site (MOp or MOs) for each Cre mouse line (Ntsr1-Cre and Rbp4-Cre) (Supplementary Movie S1).
The embryonic progeny cells of NB3-3 form an elongated cluster in the ventrolateral cortex, exclusively consisting of interneurons, which generate a projection through the anterior commissure (Fig. 6C, arrowhead).
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Furthermore, they generate a thinner projection through aV (Fig. 6H, hollow arrowhead).
They generate a single projection, which bifurcates when reaching the ventral neuropil.
The secondary cells of NB2-2 in the larva generate a single projection through aI, which stops shortly after the midline (Fig. 4D, hollow arrowhead).
ImageJ was used to generate a maximum projection for each sample and all images pertaining to one experiment were loaded as separate layers into a single Adobe Photoshop CS6 file.
SLOT detects scattered laser light from the sample at two different wavelengths to generate a pair of projection image data sets.
This strategy enabled the authors to visualize the initial invasion profile using TEM (to generate a three-dimensional projection) in which the posterior end of trypomastigotes penetrates underneath HeLa cells, resulting in actin filament enrichment at the undulated cell cortex [ 86].
To allow visualization of the arborization patterns of each of the FruM/OA neuron in three-dimensions, we incorporated the data generated from the confocal image stacks of each neuron onto a single standard brain and generated a rotating projection (Figure 4A4, B1 3 and Movie S7).
It generates a short projection, which enters the lnp laterally and spreads out extensively (Fig. 9B, arrowhead).
Each set of bootstrapped demographic rates was used to generate a new habitat specific projection matrix and to calculate contributions as above.
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