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Two generations of sub-NILs were genotyped: the BC6S1 generation was subject to MAS to generate a linkage map for each introgressed region (chr5 and chr11), and the BC6S2 generation was used to verify marker genotypes and select lines for phenotypic evaluation.
Therefore, RECORD was used to identify the best marker order in each linkage group and to generate a linkage map of rice.
This study employed both dominant (AFLP) and co-dominant (RFLP and SSR) markers to generate a linkage map.
In the initial attempt, 453 markers were shortlisted to generate a linkage map for the male T128 parent.
Then, the remaining markers from all the parts of a group were put together to generate a linkage map for the group.
The first objective of this study was to generate a linkage map of D. magna based on 109 VNTR marker loci.
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We generated a linkage map (Step 6) using the program MSTMap (Wu et al. 2008).
To identify the specific susceptibility genes generating a linkage signal, linkage studies are often followed by association mapping.
The only outcome that generates a linkage between the homologs is a CO event, in which exchanges of homologous chromosome arms occur.
In this study, we generated a linkage map containing 1,151,856 high quality SNPs between Mo17 and B73, which were verified in the maize intermated B73 × Mo17 (IBM) Syn10 population.
It reacts spontaneously by a Michael addition reaction with free sulfhydryl groups to generate a thioether linkage with cysteine residues in proteins.
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