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Here, we know how to generate a frame, so we can use graphics memory as the limiting resource that can dictate our design.
Since it is known that the B-spline (B_{2}) does not generate a frame for (b=2) [16] 16], we cannot go beyond this.
This electrical pulse is then used to generate a frame trigger signal by an in-house built custom circuit.
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To delete the region between the loxP sequences and to generate a frame-shift mutation of a Got1l1 allele (Got1l1 KO), a circular pCre-PAC plasmid (Taniguchi et al. 1998) was transiently introduced into the ES clone.
These site-specific nucleases are programmable, that is, they can induce DNA double-strand breaks (DSBs) that stimulate non-homologous ends-joining (NHEJ) and/or subsequent homologous recombination (HR) at targeted loci [ 14], therefore generate a frame-shift mutation or a replacement with an extragenous null allele.
In particular, Proposition 1.1 and Theorem 1.2 imply that for (Nin mathbb {N}setminus{1}), the functions (B_{N}), (f_{N}), and (h_{N}) generate frames whenever (0< a< N) and (0< bleqfrand4}{N+3a}), and (g_{N}) generates a frame whenever (frac{3N}{7}leq a< N) and (0< bleqfrac{4}{N+3a}).
This generates a frame shift resulting in a predicted premature truncation of the gene product.
We generated a conditional Lsd1 allele in which Cre-recombinase mediated exCre-recombinase mediatedgexcision a frame shift and premature stof in thexonsA.
Indeed, sequence analysis of the product revealed inclusion of intron 25 which generates a frame shift that introduces a stop six amino acids past the coding amino acid 1018 in the zebrafish transcript (Fig. 2A).
This may be due to the fact that PC-3 cells are hemizygous for chromosome 17p [ 41]; and a base pair deletion of the single copy of the p53 gene which generates a frame shift and a new in-frame stop codon may not provide the optimal hybridization between wild-type p53 probes and p53 transcripts in PC-3 cells.
A 107 bp CREE is present 44 bp into the CDS, which introduces a termination codon shortly thereafter and generates a frame-shift.
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