Sentence examples for generate a disruption from inspiring English sources

The checkerboard pattern might generate a disruption of the default visual network, with a specific activation of striatal and extra striatal cortex [6, 7] and a consequent flow of information across the brain regions, which followed a different behavior in the two types of migraine, as was also confirmed by the brain networking analysis.

We used homologous recombination to generate a ddTpp1 disruption mutant (tpp1−, see Materials and Methods) in the Ax2 wild-type background and confirmed disruption of the ddTpp1 gene by using PCR analyses (supplementary material Fig. S2).

Additionally, transposon insertion alleles can be uniquely informative: a single insertion may be sufficient to generate a gene disruption, reporter gene fusion, epitope-tagged allele, and conditional mutation.

These constructs were then PCR amplified to generate a linear disruption allele and gel purified for use in biolistic transformation into appropriate auxotroph strain [57].

To generate a ddTpp1 disruption mutant, the ddTpp1 REMI vector was linearized using EcoRI digestion and was then transformed into wild-type cells.

The Candida maltosa LEU2 sequence from plasmid pSN40 (Noble and Johnson 2005) was fused to flanking homology to the 5′ upstream and 3′ downstream regions of the TOF1 gene to generate a tof1∆:: C.m.LEU2 disruption cassette.

To generate a ddTpp1 disruption vector in which LoxP sites flank the blasticidin resistance cassette, we cloned the homologous arms from the ddTpp1 REMI vector into the vector pLPBLP (Faix et al., 2004).

The sequence of C. dubliniensis HIS1 from plasmid pSN52 (Noble and Johnson 2005) was fused to flanking homology to the 5′ upstream and 3′ downstream regions of the MMS22 gene to generate a mms22∆:: C.disruptionruption cassette.

Using a similar strategy as described for prf above, we generated a conditional disruption of the nmt gene.

Gamergate illustrates how successfully a toxic fringe can exploit mainline digital tools to generate a disproportion level of online disruption on the very mainstream channels that actively disown their views.

To generate a panel of deletion C. glabrata strains (Table 1), a set of plasmids containing disruption cassettes was generated.